RIPA Lysis Buffer System from Santa Cruz Biotechnology, Inc.

Product Specs
ItemRIPA Lysis Buffer System
CompanySanta Cruz Biotechnology, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog Numbersc-24948A
Quantity500 ml
TypeBiological Buffers

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Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) Ouabain’s Influence on TRPV4 Channels of Epithelial Cells: An Exploration of TRPV4 Activity, Expression, and Signaling PathwaysInternational Journal of Molecular SciencesNovember 24, 2023Arturo Ponce, Isabel Larre, Lidia Jimenez, Maria Luisa Roldán, Liora Shoshani, Marcelino Cereijidothen subjected to cell lysis utilizing RIPA buffer (Santa Cruz Biotechnology, Santa Cruz, CA, Cat. sc-24948A) comprising a protease inhibitor mix for 15 min at 4 °C. The resulting cell lysate was collected into

(2) Liposome-Mediated Delivery Improves the Efficacy of Lisosan G against Retinopathy in Diabetic MiceCellsOctober 13, 2023Rosario Amato, Alberto Melecchi, Laura Pucci, Alessio Canovai, Silvia Marracci, Maurizio Cammalleri, Massimo Dal Monte, Carla Caddeo, Giovanni Casini(STZ; cat. S0130) was purchased from Sigma Aldrich-Merck (St. Louis, MO, USA). RIPA lysis buffer (cat. sc-24948A) was obtained from Santa Cruz Biotechnology (Dallas, TX, USA), while the phosphatase and proteinase

(3) Investigating Carvedilol’s Repurposing for the Treatment of Non-Small Cell Lung Cancer via Aldehyde Dehydrogenase Activity Modulation in the Presence of β-Adrenergic AgonistsCurrent Issues in Molecular BiologySeptember 29, 2023Balqis A. Ikhmais, Alaa M. Hammad, Osama H. Abusara, Lama Hamadneh, Hamza Abumansour, Qasem M. Abdallah, Ali I. M. Ibrahim, Lina Elsalem, Mariam Awad, Rahaf Alshehada(Bredbury, UK). Dimethyl sulfoxide (DMSO) was purchased from TEDIA (Fairfield, OH, USA). RIPA lysis buffer (sc-24948A) was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Bicinchoninic acid (BCA) assay kit was

(4) Occludin Regulates HIV-1 Infection by Modulation of the Interferon Stimulated OAS Gene FamilyMolecular neurobiologySeptember 1, 2023Torices S, Teglas T, Naranjo O, Fattakhov N, Frydlova K, Cabrera R, Osborne OM, Sun E, Kluttz A, Toborek M.μl/well of RIPA buffer including protease inhibitors (Santa Cruz Biotechnology, Dallas, TX, USA, Cat# sc-24948a). Protein concentrations were measured by BCA Protein Assay Kit (Thermo Fisher Scientific, Cat# 23223)

(5) Levcromakalim provokes an acute rapid-onset migraine-like phenotype without inducing cortical spreading depolarizationThe Journal of Headache and PainJuly 24, 2023Berkay Alpay, Bariscan Cimen, Elif Akaydin, Hayrunnisa Bolay, Yildirim Sarag/kg) and parietal cortices were removed. Collected tissues were homogenized with RIPA lysis buffer (sc-24948A, Santa Cruz Biotechnology, Dallas, TX, USA) as indicated by the manufacturer and lysates were stored

(6) FOLR1-induced folate deficiency reduces viral replication via modulating APOBEC3 family expressionVirologica SinicaJune 1, 2023Wu J, Han Y, Lyu R, Zhang F, Jiang N, Tao H, You Q, Zhang R, Yuan M, Nawaz W, et al.shown in Supplementary Table S2. 2.6 Western blot Cells were lysed with RIPA lysis buffer (Cat. No. sc-24948A; Santa Cruz, USA) and total protein concentrations were determined by the BCA protein assay (Cat. No.

(7) Berberine Inhibited Growth and Migration of Human Colon Cancer Cell Lines by Increasing Phosphatase and Tensin and Inhibiting Aquaporins 1, 3 and 5 ExpressionsMoleculesApril 29, 2023Noor Tarawneh, Lama Hamadneh, Bashaer Abu-Irmaileh, Ziad Shraideh, Yasser Bustanji, Shtaywy Abdallahumidity for 72 h. Subsequently, total protein was extracted from cells using RIPA lysis buffer system (SC-24948A; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) supplemented with phosphatase inhibitor cocktail IV

(8) Quantitative Proteomics for the Identification of Differentially Expressed Proteins in the Extracellular Vesicles of Cervical Cancer CellsVirusesMarch 8, 2023Víctor Acevedo-Sánchez, Roy S. Martínez-Ruiz, Sergio R. Aguilar-Ruíz, Honorio Torres-Aguilar, Pedro Chávez-Olmos, Efraín Garrido, Rafael Baltiérrez-Hoyos, María de los A. Romero-TlaloliniQuantification Extracellular vesicles were lysed with a radioimmunoprecipitation assay (RIPA) buffer (sc-24948A, Santa Cruz Biotechnology), and protein concentration was determined using a Pierce BCA Protein Assay Kit

(9) In Vitro Evaluation of ALDH1A3-Affinic Compounds on Breast and Prostate Cancer Cell Lines as Single Treatments and in Combination with DoxorubicinCurrent Issues in Molecular BiologyMarch 6, 2023Osama H. Abusara, Ali I. M. Ibrahim, Hamzah Issa, Alaa M. Hammad, Worood H. IsmailPC-3 Cell Lines Samples of MCF7, MDA-MB-231, and PC-3 cells were lysed using a RIPA lysis buffer (sc-24948A, Santa Cruz Biotechnology, Dallas, TX, USA). Equal quantities of isolated proteins were then mixed with

(10) Analysis of the Role of Stellate Cell VCAM-1 in NASH Models in MiceInternational journal of molecular sciencesMarch 2, 2023Chung KJ, Legaki AI, Papadopoulos G, Gercken B, Gebler J, Schwabe RF, Chavakis T, Chatzigeorgiou A.snap frozen in liquid nitrogen and homogenized in RIPA lysis buffer (Santa Cruz, Heidelberg, Germany, sc-24948A) containing a protease and phosphatase inhibitor cocktail (Roche, 04693159001, CO-RO) by using the Precellys

(11) Disruption of adipocyte YAP improves glucose homeostasis in mice and decreases adipose tissue fibrosisMolecular MetabolismDecember 1, 2022Daniel Jong Jin Han, Rukhsana Aslam, Paraish S. Misra, Felix Chiu, Tanvi Ojha, Apu Chowdhury, Carmen K. Chan, Hoon-Ki Sung, Darren A. Yuen, Cynthia T. Lukin liquid nitrogen. Cold lysis buffer consisting of RIPA buffer and a protease inhibitor cocktail (sc-24948A; Santa Cruz Biotechnology, Dallas, TX, USA) was then added to the crushed tissues to mechanically homogenize

(12) Single-cell RNA sequencing uncovers a neuron-like macrophage subset associated with cancer painScience AdvancesOctober 7, 2022Philip Chiu-Tsun Tang, Jeff Yat-Fai Chung, Jinyue Liao, Max Kam-Kwan Chan, Alex Siu-Wing Chan, Guangyao Cheng, Chunjie Li, Xiao-Ru Huang, Calvin Sze-Hang Ng, Eric W-F Lam, et al.SEM. Western blotting The tissues or cells were lysed by RIPA Lysis Buffer System (Santa Cruz Biotechnology, sc-24948A). The protein concentration was measured by DC protein assay (Bio-Rad, #5000114), and 40 μg of protein

(13) Complement Is Required for Microbe-Driven Induction of Th17 and PeriodontitisThe Journal of ImmunologyOctober 1, 2022Hui Wang, Hidetaka Ideguchi, Tetsuhiro Kajikawa, Dimitrios C. Mastellos, John D. Lambris, George Hajishengallisdifferent time lengths indicated in figure 5F. Cell lysates were prepared using RIPA lysis buffer (cat# sc-24948A, Santa Cruz Biotechnology) and protein concentrations were determined using the Bradford dye assay (cat#

(14) Matrix produced by diseased cardiac fibroblasts affects early myotube formation and functionActa BiomaterialiaOctober 1, 2022Janny Piñeiro-Llanes, Silveli Suzuki-Hatano, Ananya Jain, Valerie A. Pérez Medina, William Todd Cade, Christina A. Pacak, Chelsey S. Simmonsradioimmunoprecipitation assay buffer (RIPA) supplemented with protease inhibitors and phosphatase inhibitors (Santa Cruz, sc-24948A). The use of RIPA buffer led to some insoluble matter left behind. Nevertheless, we decided to use RIPA

(15) Chronic stress disrupts the homeostasis and progeny progression of oligodendroglial lineage cells, associating immune oligodendrocytes with prefrontal cortex hypomyelinationMolecular PsychiatryJune 1, 2022Alexandros G. Kokkosis, Miguel M. Madeira, Matthew R. Mullahy, Stella E. Tsirkainhibitors (200 mM PMSF, 100 mM sodium orthovanadate, and protease inhibitor cocktail; Santa Cruz, sc-24948A). Lysates were shaken for 15 min at 4 °C, cleared by centrifugation at 15,000 g for 10 min at 4°C, and

(16) HMGB1 mediates invasion and PD-L1 expression through RAGE-PI3K/AKT signaling pathway in MDA-MB-231 breast cancer cellsBMC cancerMay 24, 2022Amornsupak K, Thongchot S, Thinyakul C, Box C, Hedayat S, Thuwajit P, Eccles SA, Thuwajit C.AT13148 (21597, Cayman Chemical, MI, USA) in 0.1% BSA DMEM for 30 min. Cells were lysed in RIPA buffer (sc-24948A, Santa Cruz Biotechnology) containing lysis buffer, PMSF, sodium orthovanadate, and protease inhibitor

(17) Synergistic Inhibition of Pro-Inflammatory Pathways by Ginger and Turmeric Extracts in RAW 264.7 CellsFrontiers in PharmacologyMay 19, 2022Xian Zhou, Gerald Münch, Hans Wohlmuth, Sualiha Afzal, Ming-Hui (Tim) Kao, Ahmad Al-Khazaleh, Mitchell Low, David Leach, Chun Guang Limixed with 100 μL of the ice-cold RIPA lysis buffer (Santa Cruz Biotechnology, Australia, cat. no. sc-24948A) and 0.1% of the protease/phosphatase inhibitor cocktail (Cell Signaling Technologies, United States,

(18) Proliferation and apoptosis regulation by G protein‑coupled estrogen receptor in glioblastoma C6 cellsOncology LettersMay 18, 2022Coral Estefania Gutiérrez‑Almeida, Anne Santerre, Lilia Carolina León‑Moreno, Irene Guadalupe Aguilar‑García, Rolando Castañeda‑Arellano, Sergio Horacio Dueñas‑Jiménez, Judith Marcela Dueñas‑jiméneztreatments. Cells were lysed in RIPA lysis buffer supplemented with a protease inhibitor cocktail (cat. no. sc-24948A; Santa Cruz Biotechnology, Inc.). Protein concentration was determined using the Bradford microplate

(19) Metformin Enhances TKI-Afatinib Cytotoxic Effect, Causing Downregulation of Glycolysis, Epithelial–Mesenchymal Transition, and EGFR-Signaling Pathway Activation in Lung Cancer CellsPharmaceuticals (Basel)March 21, 2022Pedro Barrios-Bernal, Norma Hernandez-Pedro, Mario Orozco-Morales, Rubí Viedma-Rodríguez, José Lucio-Lozada, Federico Avila-Moreno, Andrés F. Cardona, Rafael Rosell, Oscar Arrietacell lines were lysed using the RIPA lysis buffer system (Santa Cruz Biotechnology. Dallas, TX, USA. SC-24948A), according to the manufacturer’s instructions. Cell lysates were used to quantify proteins by Bradford

(20) Lentiviral-mediated ectopic expression of YAP and TAZ in YAPoff cancer cell linesSTAR ProtocolsDecember 17, 2021Joel D. Pearson, Rod BremnerBioproducts,Cat# 811-570-FL RIPA Lysis Buffer System (includes PMSF),Santa Cruz Biotechnology,Cat# sc-24948A Protease/Phosphatase Inhibitor Cocktail,Cell Signaling Technology,Cat# 5872S SDS (20% solution),Wisent

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RIPA Lysis Buffer System from Santa Cruz Biotechnology, Inc.

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RIPA Lysis Buffer System from Santa Cruz Biotechnology, Inc.

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